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xpc  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc xpc
    Xpc, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/xpc/XPC+Antibody/pm41285712-77-36-37
    Average 93 stars, based on 21 article reviews
    xpc - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Control:

    Article Title: The ATR Inhibitor VE-821 Enhances the Radiosensitivity and Suppresses DNA Repair Mechanisms of Human Chondrosarcoma Cells.
    Article Snippet: The proteins were separated by SDS-PAGE and were blotted on AmershamTM ProtranTM Premium 0.45 μM nitrocellulose membranes (GE Healthcare Life Science, Little Chalfont, UK). .. Primary antibodies against the DNA damage key players phospho-histone γH2AX, Rad51, p-BRCA1, p-p53, p-ATM, MSH3, XPC; the serine/threonine kinases p-CHK1 and p-CHK2; the apoptotic proteases caspase 8, caspase 9, cleaved caspase 3; the apoptosis-inhibiting protein survivin (BIRC5), and β-actin (all Cell Signaling Technology, Danvers, MA, USA) as loading control were used. ..

    Article Title: The ATR Inhibitor VE-821 Enhances the Radiosensitivity and Suppresses DNA Repair Mechanisms of Human Chondrosarcoma Cells
    Article Snippet: The proteins were separated by SDS-PAGE and were blotted on AmershamTM ProtranTM Premium 0.45 μM nitrocellulose membranes (GE Healthcare Life Science, Little Chalfont, UK). .. Primary antibodies against the DNA damage key players phospho-histone γH2AX, Rad51, p-BRCA1, p-p53, p-ATM, MSH3, XPC; the serine/threonine kinases p-CHK1 and p-CHK2; the apoptotic proteases caspase 8, caspase 9, cleaved caspase 3; the apoptosis-inhibiting protein survivin (BIRC5), and β-actin (all Cell Signaling Technology, Danvers, MA, USA) as loading control were used. ..

    Immunoprecipitation:

    Article Title: hSSB2 (NABP1) is required for the recruitment of RPA during the cellular response to DNA UV damage
    Article Snippet: Ratio of dead cells (PI positive) is calculated by using the cell viability imaging software (Cytell software, GE Healthcare). .. The antibodies against ATR (2790), Chk1 (2345), Ser345 phospho-Chk1 (2341), RPA32 (52448), XPC for immuno-precipitation assay (14768) and Histone H3 (9715) were purchased from Cell Signaling. .. IntS3 (A302-051A) and Ser33 phospho-RPA32 (A300-246A) antibodies were purchased from Bethyl Laboratories.

    Incubation:

    Article Title: Downregulation of ZFP36L1 contributes to methotrexate resistance in osteosarcoma through enhanced NHEJ DNA repair mechanisms.
    Article Snippet: Equal amounts of protein samples were separated on 8- 12% SDS‒PAGE gel and transferred to 0.22 μm or 0.45 μm polyvinylidene fluoride (PVDF) membranes (Merck Millipore, USA). .. The membranes were incubated with the selected primary antibodies against ZFP36L1 (Solarbio, #K110803P), GAPDH (CST, #2118S), c-caspase3 (CST, #9661S), caspase3 (CST, #9662S), γ-H2AX (CST, #2577S), H2AX (CST, #2595S), DCLRE1C (Santa Cruz, #sc-518193), XRCC1 (CST, # 2735S), XPC (CST, # 12701T), ZFP36 (proteintech, # 12737-1-AP), and ZFP36L2 (CST, # 85891T) at 4 °C overnight, followed by blocked with 5% skim milk. .. After incubation with horseradish peroxidase (HRP)-conjugated antibody (Boster, China), the HRP activity was visualized with Chemiluminescent HRP Substrate (Merck Millipore, USA) and detected with an ECL detection system.

    Article Title: Downregulation of ZFP36L1 contributes to methotrexate resistance in osteosarcoma through enhanced NHEJ DNA repair mechanisms
    Article Snippet: Equal amounts of protein samples were separated on 8-12% SDS‒PAGE gel and transferred to 0.22 μm or 0.45 μm polyvinylidene fluoride (PVDF) membranes (Merck Millipore, USA). .. The membranes were incubated with the selected primary antibodies against ZFP36L1 (Solarbio, #K110803P), GAPDH (CST, #2118S), c-caspase3 (CST, #9661S), caspase3 (CST, #9662S), γ-H2AX (CST, #2577S), H2AX (CST, #2595S), DCLRE1C (Santa Cruz, #sc-518193), XRCC1 (CST, # 2735S), XPC (CST, # 12701T), ZFP36 (proteintech, # 12737-1-AP), and ZFP36L2 (CST, # 85891T) at 4 °C overnight, followed by blocked with 5% skim milk. .. After incubation with horseradish peroxidase (HRP)-conjugated antibody (Boster, China), the HRP activity was visualized with Chemiluminescent HRP Substrate (Merck Millipore, USA) and detected with an ECL detection system.



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